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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Cancer Cell. 2010 Feb 17;17(2):198–212. doi: 10.1016/j.ccr.2009.12.040

Figure 2.

Figure 2

Co-localization of MLL fusion proteins and AEP components on chromatin

(A) Relative expression of various genes (indicated on the right) in seven human cell lines was analyzed by quantitative RT-PCR. Expression levels were normalized to GAPDH and depicted relative to the highest value among the seven cell lines arbitrarily set as 100. Error bars represent standard deviations of triplicate PCRs.

(B) Genomic localizations of various proteins in HB1119 cells were determined by ChIP assay. Cross-linked chromatin was immunoprecipitated with antibodies specific for the indicated proteins and analyzed by quantitative PCR using primer/probe sets that target promoter-adjacent regions or other genomic regions indicated at the bottom. Occupancies are displayed relative to the highest value in the group arbitrarily set as 100. Error bars represent standard deviations of triplicate PCRs. Genes expressed more than 20% of the highest levels in panel A are defined as active genes.

(C) A comparable analysis as (B) was performed for MV4-11 cells, which harbor a t(4;11) translocation and express MLL-AF4 proteins. The purple rectangle highlights a locus on which di-methyl H3K79 marks were absent, but the MLL-AF4/AEP complex was present.

See also Figure S2.