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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Cancer Cell. 2010 Feb 17;17(2):198–212. doi: 10.1016/j.ccr.2009.12.040

Figure 7.

Figure 7

ENL is required for MLL-AF6-dependent transactivation and transformaion

(A) The experimental scheme to evaluate the effect of Enl knockdown on MLL transformation is shown.

(B) Clonogenic potentials are shown for myeloid cells transformed by MLL oncogenes (indicated below) at the second plating after sh-RNA transduction (vector or sh-Enl). CFU numbers are displayed relative to the vector control arbitrarily set as 100. Error bars represent standard deviations of three independent analyses.

(C) MLL-AF6-transformed cells from first and second round colonies following sh-RNA transduction (vector or sh-Enl) were analyzed by RT-PCR for expression of endogenous Enl or Hoxa9. Expression levels were normalized to GAPDH levels and displayed relative to the transcript levels in vector control cells arbitrarily set as 100. Error bars represent standard deviations of triplicate PCRs.

(D) The same analysis as (C) was performed on MLL-ENL-transformed cells. Note that data in (B) and (D) are partially redundant with Figures 3I and 3J.