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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Virology. 2010 Jan 3;398(2):208–213. doi: 10.1016/j.virol.2009.12.004

FIG. 4.

FIG. 4

UL16 is packaged and associates with cytoplasmic capsids in the absence of UL21. Cytoplasmic capsids from detergent lysed HSV or ΔUL21 infected cells were sedimented in a 20 to 50% (wt/vol) sucrose gradient for 1 h. Regions of the gradient containing capsid bands were isolated with a syringe and then pelleted through a 30% (wt/vol) sucrose cushion. Virions present in the media were sucrose gradient purified as described. Virions and capsids with their associated proteins were separated by SDS-PAGE in 10% gels and analyzed by immunoblot with VP5 and UL16 specific antibodies. cyto, cytoplasmic.