Table 1.
Forward and reverse primer sequences for 5-HT-related and control transcripts
| Accession (NCBI) |
Gene | Foward / reverse primers (5′ → 3′) | Product size (bp) |
|---|---|---|---|
| NM_008308 | Htr1a | GGATGTTTTCCTGTCCTGGT / CACAAGGCCTTTCCAGAACT | 121 |
|
| |||
| NM_010482 | Htr1b | TCACATGGCCATTTTTGACT / CAGTTTGTGGAACGCTTGTT | 112 |
|
| |||
| NM_172812 | Htr2a | AGAACCCCATTCACCATAGC / ATCCTGTAGCCCGAAGACTG | 119 |
|
| |||
| NM_008312 | Htr2c | GATTGGACTGAGGGACGAAA / ATGAAGAATGCCACGAAGGA | 102 |
|
| |||
| NM_013561 | Htr3a | CTTCCCCTTTGATGTGCAG / CCACTCGCCCTGATTTATG | 139 |
|
| |||
| NM_021358 | Htr6 | CATAGCTCAGGCCGTATGTG / CGCATGAAGAGGGGATAGA | 111 |
|
| |||
| NM_008315 | Htr7 | TTCTGCAACGTCTTCATCG / ATTCTGCCTCACGGGGTA | 126 |
|
| |||
| NM_010484 | Slc6a4 | CAACTCCGGCTTTTCCAATA / ATTTCCGTTGGTGTTTCAGG | 176 |
| NM_173391 | Tph2 | TGCAAGCAAGAGGGTCAACT /CATGCTTCAATTCTCCGATG | 70 |
| NM_008907 | Ppia | AATGCTGGACCAAACACAAA / CCTTCTTTCACCTTCCCAAA | 101 |
|
| |||
| NM_013556 | Hprt1 | TGTTGTTGGATATGCCCTTG / GCGCTCATCTTAGGCTTTGT | 106 |
|
| |||
| NM_008084 | Gapdh | AGGAGCGAGACCCCACTAAC / GTGGTTCACACCCATCACAA | 179 |
|
| |||
| NM_007393 | Actb | GTGGGAATGGGTCAGAAGG / GGTCATCTTTTCACGGTTGG | 228 |