Figure 1.

Rapamycin Treatment of Adult Drosophila Downregulates TOR Activity

(A) Western blot analysis of phospho-S6K on whole-fly protein extracts. Flies were sampled after 1, 3, 5, or 12 days of rapamycin treatment at concentrations of 200 or 400 μM. A dose-dependent reduction in phospho-T398-S6K levels was observed and the degree of inhibition increased with longer treatment time. For all western blots, relative band intensity was estimated using Image J.

(B) Western blot analysis of phospho-S6K in different body parts of w^Dah flies. Flies were maintained with or without 200 μM rapamycin for 2 weeks prior to preparation of protein extracts from heads, thoraces, and abdominal segments. Rapamycin was found to efficiently reduce levels of phospho-T398-S6K in all fly body parts.