Figure 2.

Effects of Rapamycin on Life Span and Fecundity in Various Standard Laboratory Strains

(A) Rapamycin treatment extends the life span of w^Dah females. Compared to flies on control food (0 μM rapamycin), flies on 50 and 200 μM rapamycin food have increased median life spans (p = 0.0005 and p < 0.0001, log-rank test).

(B) Rapamycin treatment at 50, 200, and 400 μM extends the life span of w^Dah females (p = 0.0428, p < 0.0001, p = 0.0013, log-rank test compared to control).

(C) Rapamycin extends life span in w^Dah males (p = 0.0241, log-rank test).

(D) Reduced fecundity of females on 50, 200, and 400 μM rapamycin food. Data are given as mean number of eggs laid per female per day ± SEM. T test p values between egg laying under different conditions are p < 0.005. For each time point and each condition, eggs from ten vials containing ten flies per vial were counted.

(E) Rapamycin enhances starvation resistance (p < 0.0001, log-rank test). For all stress assays, w^Dah females were pretreated for 2 weeks with 200 μM rapamycin, and initial population was 100 flies per condition.

(F) Rapamycin pretreated w^Dah females have improved survival on paraquat (p < 0.0001, log-rank test).

(G) Rapamycin treatment increases TAG levels. TAG levels in w^Dah flies were measured after 14 days of rapamycin treatment from heads and thoraces of five females. Twelve samples were measured per treatment. Data are shown as mean ± SEM. ^∗ indicates statistical significance of difference between untreated and rapamycin-treated flies (t test, p < 0.05).