Figure 6.

Effect of Rapamycin Treatment on Long-Lived IIS Mutants

(A) Rapamycin extends the life span of long-lived chico¹ heterozygotes, (p = 0.0005) but decreases the life span of chico¹/chico¹ homozygotes (p < 0.0001). On control food, chico¹ heterozygotes (p = 0.039) and chico¹/chico¹ nulls (p < 0.0001) have increased median life span compared to control w^Dah flies. Log-rank test is used for analyses.

(B) Rapamycin does not extend the life span of mNSC-ablated flies. mNSCs were partially ablated by driving expression of the proapoptotic factor reaper (UAS-rpr) in the mNSCs using the dilp2-GAL4 driver (d2GAL4). On control food, mNSC-ablated flies are long lived (p < 0.0001, log-rank test compared to control). Log-rank test p values for rapamycin-treated versus nontreated flies of the same genotype are p < 0.0001 for w^Dah and p = 0.97 for d2GAL4 > UAS-rpr.

(C) Flies pretreated with 200 μM rapamycin survived longer under starvation conditions compared to untreated flies. Log-rank statistics for nontreated versus rapamycin-pretreated flies for all genotypes are p < 0.0001. Compared to wild-type controls, both chico¹ heterozygotes and mNSC-ablated flies were more resistant to starvation without rapamycin pretreatment (p = 0.006 and p = 0.001, respectively, log-rank tests).

(D) Flies pretreated with 200 μM rapamycin survive longer when fed 20 mM paraquat. Log-rank statistics for nontreated versus rapamycin pretreated flies for all genotypes are p < 0.0001. Compared to wild-type controls, mNSC-ablated flies were more resistant to paraquat ingestion without rapamycin pretreatment (p < 0.0001, log-rank test).