Table 1. Classification of genes with respect to antisense tags.

We classified only those genes whose sum of distinct sense and antisense tags was five or more. S genes contained only sense tags or had a sense/antisense tag ratio of five or more; AS genes contained only antisense tags or had a sense/antisense tag ratio of 0.2 or less; SAS genes contained both sense and antisense tags and had a sense/antisense ratio between 0.2 and 5. Samples were derived from the following sources: PBMC, peripheral blood mononuclear cells isolated from a healthy volunteer; Jurkat, a T-cell leukemia line; HCT116, a colorectal cancer cell line; MiaPaCa2, a pancreatic cancer line; MRC5, fibroblast cell line derived from normal lung.

Cell type:		PBMC		Jurkat		HCT116		MiaPaCa2		MRC5
All genes		# of genes	Fraction	# of genes	Fraction	# of genes	Fraction	# of genes	Fraction	# of genes	Fraction
S genes		10586	81.60%	9928	89.60%	11176	88.00%	9500	89.50%	10165	89.30%
AS genes		329	2.50%	240	2.20%	203	1.60%	155	1.50%	212	1.9%
SAS genes		2061	15.9%	908	8.2%	1327	10.4%	959	9%	1002	8.8%
Total		12976		11076		12706		10614		11379
Coding genes		# of genes	Fraction	# of genes	Fraction	# of genes	Fraction	# of genes	Fraction	# of genes	Fraction
S genes		10375	81.30%	9778	89.50%	10770	87.60%	9348	89.40%	10029	89.20%
AS genes		325	2.50%	239	2.20%	201	1.60%	154	1.50%	210	2%
SAS genes		2055	16.1%	907	8.3%	1325	10.8%	959	9.2%	1000	8.9%
Total		12755		10924		12296		10461		11239
Noncoding genes		# of genes	Fraction	# of genes	Fraction	# of genes	Fraction	# of genes	Fraction	# of genes	Fraction
S genes		211	95.50%	150	98.70%	406	99.00%	152	99.30%	136	97.10%
AS genes		4	1.80%	1	0.70%	2	0.50%	1	0.70%	2	1.4%
SAS genes		6	2.7%	1	0.70%	2	0.50%	0	0%	2	1.4%
Total		221		152		410		153		140