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. 2008 Sep 1;189(1-4):105–110. doi: 10.1159/000152912

Table 1.

Primers designed for the detection and quantification of NFI-C alternative splicing

Primer sets Sequences (5_ to 3_) Expected products, bp
PCR exon 1A F: TCCTCGCAGCAGCGCCATG 534
R: TAGGCCAGGTAGAGGTCCAG
PCR exon 1B F: ATGACTCAGTAAGTTCAGCGC 606
R: TAGGCCAGGTAGAGGTCCAG
PCR NFI-C1/C2/C4 F: CTGGTCATGGTCATCCTGTTC 390
  NFI-C3 R: GTTCAGGTCGTATGCCAGGT 318
PCR NFI-C1 F: GCTCTGCATTTCCCTACGAC 456
  NFI-C2/C3 R: TTCCTGGGACGATGGAGAAG 302
 NFI-C4 216
qRT-PCR exon 1A F: TCCTCGCAGCAGCGCCATG 300
R: TTCTTGCCGGTGATGCTCAGCA
qRT-PCR exon 1B F: ATGACTCAGTAAGTTCAGCGC 280
R: CCCACTTCTGCTTGACCTC
qRT-PCR NFI-C2 F: CAGCAACCTGGACCGCCT 161
R: TTCCTGGGACGATGGAGAAG
qRT-PCR NFI-C4 F: CAGCAACCTGGACCGTCC 75
R: TTCCTGGGACGATGGAGAAG
qRT-PCR NFI-C1 F: ACCACCTCCGAGGGAGGA 186
R: TTCCTGGGACGATGGAGAAG

F = Forward; R = reverse; qRT-PCR = quantitative real-time PCR.