Table 1.
Primer sets | Sequences (5_ to 3_) | Expected products, bp | |
---|---|---|---|
PCR exon 1A | F: | TCCTCGCAGCAGCGCCATG | 534 |
R: | TAGGCCAGGTAGAGGTCCAG | ||
PCR exon 1B | F: | ATGACTCAGTAAGTTCAGCGC | 606 |
R: | TAGGCCAGGTAGAGGTCCAG | ||
PCR NFI-C1/C2/C4 | F: | CTGGTCATGGTCATCCTGTTC | 390 |
NFI-C3 | R: | GTTCAGGTCGTATGCCAGGT | 318 |
PCR NFI-C1 | F: | GCTCTGCATTTCCCTACGAC | 456 |
NFI-C2/C3 | R: | TTCCTGGGACGATGGAGAAG | 302 |
NFI-C4 | 216 | ||
qRT-PCR exon 1A | F: | TCCTCGCAGCAGCGCCATG | 300 |
R: | TTCTTGCCGGTGATGCTCAGCA | ||
qRT-PCR exon 1B | F: | ATGACTCAGTAAGTTCAGCGC | 280 |
R: | CCCACTTCTGCTTGACCTC | ||
qRT-PCR NFI-C2 | F: | CAGCAACCTGGACCGCCT | 161 |
R: | TTCCTGGGACGATGGAGAAG | ||
qRT-PCR NFI-C4 | F: | CAGCAACCTGGACCGTCC | 75 |
R: | TTCCTGGGACGATGGAGAAG | ||
qRT-PCR NFI-C1 | F: | ACCACCTCCGAGGGAGGA | 186 |
R: | TTCCTGGGACGATGGAGAAG |
F = Forward; R = reverse; qRT-PCR = quantitative real-time PCR.