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. 2008 Aug 26;189(1-4):158–162. doi: 10.1159/000151725

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Copyright © 2008 by S. Karger AG, Basel

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Fig. 1. — The effect of ANK on osteoblastic differentiation of MC3T3-E1 cell line. a Immunoblot analysis of ANK protein expression in nontransfected (NT), control siRNA-transfected (Ctrl siRNA) and ank-specific siRNA-transfected (ank siRNA) MC3T3-E1 cells after 3 days of transfection. b Gene expression of APase, type I collagen [α1(I)], bone sialoprotein (BSP), osteocalcin (OC), osterix and runx2 in nontransfected (NT), control siRNA-transfected (Ctrl siRNA) and ank-specific siRNA-transfected (ank siRNA) MC3T3-E1 cells after 8 days of transfection. Gene expression of APase, α1(I), BSP, OC and osterix was detected using quantitative real-time PCR analysis and SYBR Green.