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Different affinities of mutated and wild-type amelogenins for hydroxyapatite. Wild-type amelogenin (rh174) or mutants (P156T or P164T) were mixed with HAP with constant shaking. Both P156T and P164T amelogenin mutants have significantly reduced protein-absorbed amount per square meter of HAP, compared to the wild-type controls. * p < 0.001.
