FIGURE 2.

ITC measurements of the binding of heparin to Tau_244–372 and the fibrillation of Tau_244–372 induced by heparin at 37.0 °C. A represents typical calorimetric titration of Tau_244–372 (40 μm) with heparin (500 μm) in the absence of DTT. B shows the plots of the heat evolved (kcal) per mol of heparin added, corrected for the heat of heparin, against the molar ratio of heparin to Tau_244–372. The data (solid squares) were fitted to a single set of identical sites model, and the solid line represented the best fit. C represents typical calorimetric curve for the fibrillation of Tau_244–372 induced by heparin as a function of time following the binding of heparin to Tau_244–372, and the inset shows the heat effect of the fibrillation of Tau_244–372. D represents the transmission electron micrographs of Tau_244–372 fibrils formed in the calorimeter cell. The scale bar represents 200 nm.