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Physical interaction between Rtt106p-M and histones. A, Ni-NTA pull-down analysis of Rtt106p-M with histones H3-H4 and H2A-H2B in cell lysates. B, highlighted are the mutated residues in Rtt106p-M loop mutants. C, the CD spectra of Rtt106p-M and its mutants. D, Ni-NTA pull-down analysis of Rtt106p-M mutants with histones H3-H4 in cell lysates. The final samples were resolved by Tricine-SDS-PAGE (15%, w/v) and stained with Coomassie Brilliant Blue (CB).
