FIG. 5.

Insulin-like growth factor-1 receptor (IGF-1R) immunohistochemical staining of the ipsilateral hemisphere after controlled cortical impact (CCI) brain injury. These images show sham-injured mouse cortex (a), subcortical white matter (SCWM) (e), and thalamus (g). IGF-1R staining in the sham-injured cortex was predominantly in neuron-like cells (inset in a), with slightly higher staining intensity in cortical layer IV (a). At 1 h post-injury, IGF-1R staining was most decreased at the contusion borders (marked by the asterisk; lesion center to the left), but was relatively preserved in layer IV (b). Many intensely-stained cells were seen in the contusion periphery at 48 h (c) and at 72 h (d), in combination with IGF-1R-positive blood vessels (arrows in c and d; lesion cavity is at left). The ipsilateral SCWM exhibited increased IGF-1R staining at 48 h after CCI (f ). In the ventral thalamic nucleus, receptor staining was increased in neurons at 72 h after CCI (h) (scale bar in d = 100 μm [a–f are same magnification]; scale bar in h = 50 μm [g and h are same magnification]; scale bar in inset in a = 20 μm).