FIG. 9.

Effect of delayed treatment with SNX-185 on neuron survival. Neurons grown in mixed neuronal–glial cell cultures were treated with 100 nM or 1000 nM SNX-185 5 min after mild, moderate, or severe stretch injury and returned to a cell incubator for a 24-h recovery period. Cells were then loaded with carboxyfluorescein diacetate (stains viable cells) and propidium iodide (stains non-viable cells) and visualized with a 400 × upright microscope. Cell counts were performed in five high-power fields (hpf ) per well. Delayed post-injury treatment with 100 or 1000 nM SNX-185 did not significantly affect the number of viable, surviving neurons (A). The number of non-viable, propidium iodide-positive neurons per 5 hpf (B) was significantly increased with delayed 100 (n = 6, p < 0.01) or 1000 nM (n = 6, p < 0.05) SNX-185 treatment following mild injury and significantly decreased when given at 1000 nM 5 min after severe injury (n = 6, p < 0.05). *Statistically significant at p < 0.05.