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The manual (black) and threshold (white) segmentation of normal-appearing VLWM in Asd (a) and AI (b) maps of a control cord. Two normal distributions of the pixel numbers of anisotropy were observed in manually defined GM and VLWM in Asd (c) and AI (d) map. The mean ± 2 × SD (95.4% confidence interval) of VLWM was used as the threshold to define the region of normal-appearing VLWM in the control and injured spinal cords, designated as ROIAsd and ROIAI.
