Fig. 3.
Genetic variation in HAS2 is associated with skin wrinkling in Shar-Pei. (A) Single locus estimates of FST between Shar-Pei and Dachshund across a 1-Mb window. Similar patterns were observed for Shar-Pei compared to other breeds, but have been omitted for clarity. The location of all protein-coding genes are shown as rectangular boxes. (B) An example of smooth (Left) and wrinkled (Right) Shar-Pei dogs. (C) Exon structure of HAS2. Conservation values obtained from the University of California Santa Cruz genome browser are shown below. Black horizontal lines indicate sequenced regions. (D) Genotype frequencies of the intron 2 indel (site 13805 in Table S4) in smooth and wrinkled Shar-Pei, which are significantly different (P = 6.28 × 10−5). Deletion and insertion alleles are denoted as “D” and “d,” respectively.