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EPSPS genomic copy number and glyphosate resistance cosegregate in pseudo-F2 A. palmeri populations. EPSPS copy number relative to ALS and accumulation of shikimate were determined as described in Materials and Methods. Insets: Relative copy number histograms in pseudo-F2 populations generated using (A) hand pollination (F1 male parent 18 relative EPSPS copies and F1 female parent 39 relative EPSPS copies) and (B) open pollination (parental relative copy number not measured).
