Fig. 5.

Increased EPSPS enzyme activity is positively correlated with EPSPS relative genomic copy number in four pseudo-F₂ A. palmeri plants. Glyphosate inhibition assays were normalized for TSP quantity. Data points are means and standard errors of three replications. Filled circles: 54 relative EPSPS copies, IC₅₀ (glyphosate concentration that reduced enzyme activity by 50%) = 22 μM; open circles: 39 relative copies, IC₅₀ = 15 μM; filled triangles: 8 relative copies, IC₅₀ = 36 μM; open triangles: 1 relative copy, IC₅₀ = 66 μM.