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Particle stability and binding. (A) In vitro stability against salt. Unmodified 50-nm AuNPs (I) showed instant aggregation after the addition of PBS at the fourth minute, but II–V remained stable. (B) In vitro stability against serum. PEGylation attenuated particle aggregation in 90% mouse serum. (C) Saturation binding of Tf-PEG-AuNPs to Neuro2A cells. Tf-PEG-AuNPs bound in a Tf content-dependent manner (III–V). Error bars indicate 1 SD from duplicate experiments.
