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. 2010 Jan 11;107(3):1178–1183. doi: 10.1073/pnas.0908882107

Fig. 3.

Fig. 3.

Colchicine selection increased the number of ANP-positive cells as well as the amount of pro-ANP secreted into culture medium. (A) An ANP RIA was used to measure the amount of human ANP secreted by genetically modified HSE cultured with (solid bar) or without (open bar) colchicine treatment (10 ng/mL). Determinations were made in duplicate and were repeated multiple times with similar results. (B) cGMP assay of pro-ANP levels in culture medium from genetically modified HSE with (solid bar) and without (open bar) preincubation with the corin-expressing NCI-H1688 cell line that can convert pro-ANP into active α-ANP. Ten nM vasopressin (VP) was used as a negative control, and 1 mM sodium nitroprusside (SP) was used as a positive control. (C) ANP localization in genetically modified HSE that were selected with colchicine (+ col) or were not selected (− col) using human anti-ANP antibody. Arrows indicate Fb expressing ANP.