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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: Cancer Res. 2008 Jul 15;68(14):5619–5627. doi: 10.1158/0008-5472.CAN-08-1274

Figure 1. PR-Cre efficiently deletes endometrial Pten and p53.

Figure 1

A) Western blot analysis of Pten, pAkt, and total Akt in wild-type and Ptenpr−/− uteri (upper panel). Immunohistochemistry of Pten and pAkt in 10-day (10 d) and 3-week (3 wk) old Ptenpr−/− and wild-type uteri (lower panel). Bar, 200 μm. le, luminal epithelium; ge, glandular epithelium; s, stroma; myo, myometrium. B) Western blot analysis of p53, Pten, pAkt, and total Akt in wild-type and Ptenpr−/−/p53pr−/− uteri (left panel). Actin serves as a loading control. In situ hybridization of p53 in 3-week old wild-type and Ptenpr−/−/p53pr−/− uteri (right panel). Because of extremely tiny sizes of uteri, all wild-type and Ptenpr−/− uteri from 10-day 3-week old mice were placed into small grooves made in kidney slices to serve as cassettes for making frozen sections. Kid, kidney; Bar, 400 μm. C) RT-PCR of p53 in wild-type and p53pr−/− uteri. β-Actin is a house keeping gene.

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