Figure 3. Heightened Cox-2 expression in Ptenpr−/− uteri with EMC.
A) Western blot analysis of Cox-2 and Cox-1 in Ptenpr−/− uteri. Actin serves as control. B) RT-PCR of Cox-2 in Ptenpr−/− uteri. β-Actin is a housekeeping gene. C) In situ hybridization of Cox-2 in Ptenpr−/− and wild-type uteri. Bar, 400 μm. le, luminal epithelium; ge, glandular epithelium; s, stroma; myo, myometrium; Kid, kidney. D) Immunohistochemistry of Cox-2 in uteri of 3-week old Ptenpr−/− and wild-type mice. Bar, 400 μm.