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. 2010 Jan 6;107(4):1343–1348. doi: 10.1073/pnas.0910383107

Fig. 1.

Fig. 1.

Simultaneous stable integration of two piggyBac transposons. Luciferase assay comparing the triple transfection of plasmids encoding the piggyBac transposase (pCMV-piggyBac) and pT-NeomycinR with either a plasmid encoding a luciferase cDNA (pCAGGS-Luciferase, Left) or a luciferase transposon (pT-Luciferase) (Right). Transfected HEK-293 cells were cultured for 2–3 weeks with G418 to select for stable pT-NeomycinR expression and then assayed for luciferase expression (Top). Following imaging, plates were stained with methylene blue to visualize the cells on the plates (Bottom). Shown are images representative of three independent experiments.