Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jan 6;107(4):1343–1348. doi: 10.1073/pnas.0910383107

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

PMC Copyright notice

Fig. 3. — Creation of a SCN1A stable cell line by simultaneous genomic integration of two piggyBac transposons. Efficient stable integration of sodium channel transgenes by piggyBac transposition. (A) Methylene blue staining of HEK-293 colonies following transfection with the indicated plasmids and selection with G418/Puromycin for 3 weeks. Colonies stably coexpressing pT-SCN1A:3XFLAG and pT-SCN1B:cMyc-IRES-SCN2B:HA were able to grow in the presence of G418/Puromycin selection. (B) Protein expression from each piggyBac transposon confirmed for ten selected clones by Western blot analysis. Transgene specific proteins were detected by primary antibodies directed against the appropriate epitope tag (FLAG, cMyc, or HA). Detection of the endogenous protein transferrin confirms protein loading.