Fig. 4.

MA-bound RNA suppresses membrane binding of Gag by inhibiting interactions between basic residues and acidic lipids and by reducing the myristate-dependent interaction. (A) Liposome binding of WT and delNC Gag was analyzed as in Fig. 2 by using PC+PS liposomes. Data from three independent experiments are shown as means ± SD. **, P < 0.01. There is no statistically significant difference in membrane binding of RNase-treated Gag between WT and delNC. (B and C) Liposome binding of WT and 1GA Gag was analyzed as in Fig. 2 by using liposomes containing either PC+PS or PC+PS+PI(4,5)P₂ (B) or only PC (C). Data from three to five independent experiments are shown as means ± SD. ns, not significant; *, P < 0.05; **, P < 0.01. There is a statistically significant difference (*) in binding to PC+PS liposomes but not PI(4,5)P₂-containing liposomes between WT and 1GA Gag treated with RNase.