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. 2009 Dec 28;107(4):1630–1635. doi: 10.1073/pnas.0908953107

Fig. 2.

Fig. 2.

Down-regulation of APP and SOD1 expression in DS fibroblasts by siRNA and shRNA leads to reversal of endocytic dysfunction. (A) C1/6.1 immunolabeling shows DS fibroblasts transfected with siNC and siAPP. (B) Western blot analysis shows expression of APP in siAPP-treated DS fibroblasts. (C) Immunolabeling and (D) Western blot analysis with anti-SOD1 shows expression of SOD1 in siSOD1-transfected DS fibroblasts. (E) Representative Western blot analysis of transferrin uptake at 5 and 20 min in siAPP-treated DS fibroblasts. (F) Mean level of transferrin uptake from 3 experiments. *, P < 0.05. Expression of APP is shown in DS fibroblasts treated with shAPP (G) and vector (H). Expression of SOD1 is shown in shSOD1- (I) and vector- (J) transfected DS fibroblasts. In J the brightness levels of the red channel have been adjusted.