Fig. 1.

p53 transactivation from noncanonical 1/2 sites in human cells. Sequence dependence of p53 transactivation from various 1/2 site p53 REs. Luciferase assays were performed 48 h after transfection of SaOS2 cells with the reporter constructs along with plasmids expressing WT p53 (A) or different p53 mutants (B). The original p53 1/2 site associated with FLT1 gene is indicated by the *. For evaluation of mutant p53 transactivation, the constructs designated 1/2 RE1 and RE2 were used as well as the full site p21 5′RE. Results are reported as average-fold luciferase induction relative to the empty pGL4.26 vector ± SD (n = 3).