Fig. 5.

ERβ cooperates with mutant p53 to induce transcription from the endogenous FLT1 gene. HCT116 p53^−/− cells cultured in normal media (gray bars), in estrogen-reduced media alone (black bars), or in the presence of 10⁻⁹ M of E2 (strip bars) were transiently transfected with WT or mutant p53 expression vector. After 24 h, the relative expression of the FLT1 mRNA was measured by real time PCR. The HCT116 cells are heterozygous for the p53 target 1/2 site in the FLT1 where p53 induction leads to specific induction of the T allele (11). Results are reported as average-fold ± SD (n = 3) of induction relative to the housekeeping gene β2 microglobulin.