Table 1.

Transactivation by WT and mutant p53 at 1/2 and full sites in the FLT1-T motif, alone and at endogenous FLT1-T

	Full and 1/2 site REs within1 Kb Flt1 motif::LUC										1/2 site::LUC		Endogenous FLT1-T
	Full sites						1/2 sites
	AIP		GADD45		p21		FLT1-T		1/2 RE1		1/2 RE1		1/2 RE2
p53 mutation	−ERα	+ERα	−ERα	+ERα	−ERα	+ERα	−ERα	+ERα	−ERα	+ERα	−ERα	−ERα	−ERβ	+ERβ
WT	Yes	YES**	Yes	YES**	Yes	YES**	Yes	YES**	Yes	YES**	Yes	Yes	Low	YES**
S121F	Yes	YES**	Yes	YES**	Slight	Slight	Yes	YES**	Yes	YES**	Yes	Yes	Slight	YES**
A138V	Slight	YES**	Yes	YES**	Yes	YES**	No	YES**	Yes	YES**	Yes	Yes	No	YES**
G279R	No	No	Slight	YES**	Yes	YES**	No	YES**	Slight	YES**	Yes	Yes	Slight	YES**
R337H	Slight	YES**	Yes	YES**	Yes	YES**	No	YES**	Yes	YES**	Yes	Yes	Low	YES**
E285K	No	No	Yes	YES**	No	No	No	No	Slight	Slight	Yes	No	No	No
R337C	No	No	Slight	YES**	Yes	YES**	No	No	No	No	No	No	No	No
T125R	No	No	Yes	Yes	Slight	Yes	No	No	Yes	Slight	Yes	No	No	No
Y220C	No	No	No	No	No	No	No	Slight	No	Slight	Slight	No	No	Low
M237I	No	No	No	Yes	No	No	No	No	Slight	Slight	Slight	No	Slight	No

YES**, transactivation by WT or mutant p53 is enhanced by ERα or ERβ.

*R175H, G245 and G279E were also examined (Fig. 1B) but exhibited no transactivation under any condition.