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. 2009 Dec 30;107(4):1535–1540. doi: 10.1073/pnas.0909479107

Fig. 4.

Fig. 4.

BbGL-2 binding to mCD1d. (A) Overview of mCD1d-BbGL-2c crystal structure. N-linked glycosylation of mCD1d is shown as green sticks. (B) Structures of α-galactosyl containing sphingolipids (α-GalCer and GalA-GSL) and diacylglycerolipid (BbGL-2c and -2f) iNKT cell antigens. Orientations of BbGL-2c (C) and BbGL-2f (D) in mCD1d binding groove are depicted. View of final 2FoFc map drawn as a blue mesh around BbGL-2 antigens (yellow) and contoured at 1.0σ contour level. The α2-helix is removed for clarity. Individual cis-unsaturations of alkyl chains are indicated in green. Note that electron density is fairly weak for BbGL-2f headgroup, reflecting less than full occupancy or increased binding flexibility, whereas lipid backbone binding is very well defined. Also note how sn-1 and sn-2 alkyl chains of BbGL-2c and -2f are bound in opposite orientations inside mCD1d groove.