Fig. 4.

Role of LGP2 in the recognition of exogenously transfected RNAs. (A) WT and Lgp2 ^−/− MEFs were stimulated with triphosphate RNA, in vitro–transcribed dsRNA (1 μg/mL) or poly I:C complexed with Lipofectamine 2000 for 24 h. The IFN-β concentrations in the culture supernatants were measured by ELISA. med, medium; N.D., not detected. Data are shown as the means ± SD of triplicate samples. Similar results were obtained in three independent experiments. (B) WT and Lgp2 ^−/− MEFs were transfected with the indicated amounts of poly I:C complexed with Lipofectamine 2000. The IFN-β concentrations in the culture supernatants were measured by ELISA.