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. 2010 Jan 8;107(4):1512–1517. doi: 10.1073/pnas.0912986107

Fig. 5.

Fig. 5.

Essential role of the LGP2 ATPase activity in the recognition of RNA viruses. (A) Lgp2 −/− MEFs were transiently transfected with the IFN-β promoter construct together with expression plasmids encoding LGP2 or LGP2 (K30A). The cells were infected with EMCV for 8 h and then lysed. The cell lysates were analyzed by a luciferase assay. (B) WT and Lgp2 −/− MEFs were infected with retroviruses expressing LGP2 or LGP2 (K30A). At 2 days after infection, the cells were exposed to EMCV for 24 h and the IFN-β concentrations in the culture supernatants were measured by ELISA. (C and D) WT and Lgp2 K30A/K30A (K30A) mice were exposed to the indicated RNA viruses for 24 h. The concentrations of IFN-β (C) and IL-6 (D) in the culture supernatants were measured by ELISA. (E) WT and Lgp2 K30A/K30A cDCs were transfected with the indicated RNAs for 24 h. The concentrations of IFN-β in the culture supernatants were measured by ELISA. moi, multiplicity of infection; med, medium alone; LF, lipofectamine alone. Data are shown as means ± SD and are representative of at least three independent experiments.