Fig. 2.

Suppression of proliferation and neuronal differentiation following down-regulation of miR-18 in NB cells. (A) Cell proliferation as measured by EdU incorporation of Kelly cells transfected with LNA-inhibitors for miR-18a (LNA-18a), miR-19a (LNA-19a), or control (LNA-scramble). One representative FACS plot from four independent experiments is shown; the graph is the summary of all four experiments. (B) SK-N-BE(2) cells were transduced with lentiviral constructs expressing scramble or anti-miR-18a. (Upper) Phase contrast images. (Lower) GFP expression for identification of the transduced cells. (C) qPCR analysis of the neuronal differentiation markers SCG10, GAP43, and NPY in SK-N-BE(2) cells transduced with lenti-scramble or lenti-anti-miR-18a.