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Muscarinic inhibition of D1 dopamine receptor-stimulated cAMP accumulation is abolished in the striatum of D1–M4-KO mice. cAMP assays were performed using striatal membranes prepared from D1–M4-KO and control mice by applying increasing concentrations of the D1 receptor agonist, SKF82958, either in the absence or in the presence of 100 μm carbachol (CCh). Data are given as means ± SEM (n = 3).
