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. 2009 Nov 19;29(2):376–386. doi: 10.1038/emboj.2009.342

Figure 5.

Figure 5

NUAK1 regulates the DNA content. (A) At 10 days after infection with a pLPC or a pLPC/NUAK1flag vector and puromycin selection, WI-38 cells were fixed with ethanol, stained with propidium iodide, and analysed using FACS to determine the DNA content. (B) Cell extracts were prepared from early-passage (e.p.) pLPC-infected and pLPC/NUAK1-infected and late-passage (l.p.) pRS- and pRS/NUAK1-infected WI-38 cells. Levels of the cyclin A and LATS1 proteins were checked using immunoblotting and actin level was used as a loading control. (C) WI-38 cells were fixed with PFA and stained with Hoechst. Percentages of polynucleated cells were estimated and Hoechst-stained images are shown. (D) Chromosome spreading experiments. At 7 days after infection and selection, cells were treated with colcemid. Cell images with normal and aberrant chromosome numbers are shown.