Figure 2.

Membrane-bound Arf1-GTP does not alter the ability of ALPS and ArfGAP1 to interact specifically with curved membranes. Kinesin-pulled tube networks were first incubated with 0.5 μM Arf1-OG+GTP (green). Then 3 μM ALPS1-ALPS2-Alexa⁵⁴⁶ (A) or 3 μM antibody-labelled ArfGAP1 (B) was injected (red). The lipid marker SQUARE-685-PC is shown in (B) (blue channel). Fluorescence intensity profiles in the tube and GUV regions across the dashed lines are plotted on the right panels (green line: Arf1-OG fluorescence; red line: ALPS1-ALPS2-Alexa⁵⁴⁶ fluorescence in (A) or Cy3 antibody-labelled ArfGAP1 in (B); blue line: lipid marker fluorescence). Scale bar: 15 μm.