Figure 1.

A genome-wide RNAi screen for proteins required for secretion. (A) Schematic diagram of the reporter construct used to generate the stable S2 cell line used for the screen. The metallothionein promoter and the BiP signal peptide are from Drosophila. (B) Summary of screening protocol. Secretory pathway function was quantified by determining the proportion of total luciferase activity in the well that was in the medium fraction (i.e. A/A+B). (C) Distribution of the functions of the 108 hits from the screen (for more details see Supplementary Table 1).