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. 2009 Nov 26;29(2):304–314. doi: 10.1038/emboj.2009.350

Figure 6.

Figure 6

Depletion of C4orf41 fragments the Golgi ribbon and prevents secretory cargo moving beyond the Golgi fragments. (A) Wide-field micrographs of HeLaM cells treated with the indicated siRNAs and stained for the Golgi markers GM130 or TGN46, and with DAPI to visualise the nucleus (blue). Quantitation of mock-, TMEM167-, and C4orf41-treated cells revealed perturbation of GM130 in 0, 0, and 93% respectively (n=70), and of TGN46 in 0, 5, and 95% respectively (n=110). (B) Wide-field micrographs of C1 cells treated with siRNA against C4orf41 and then fixed 80 min after induction of the release of GFP-FM4-hGH from the ER as in Figure 4. The cells were labelled with the indicated markers for the cis-Golgi (GM130), the trans-Golgi (golgin-245), and lysosomes (Lamp II). To confirm the Golgi fragmentation phenotype, we performed each staining experiment with two different oligonucleotides against C4orf41, and representative results are shown.