Fig. 1.
Expression of the α6 integrin, but not α6p, was dependent on translation. Human prostate carcinoma DU145H cells were treated with either 2.25 μM puromycin or DMSO vehicle for 18 h. Whole cell lysate (10 –15 μg) was loaded and electrophoresed on a 7.5% polyacrylamide gel under nonreducing conditions. Proteins were transferred to PVDF membrane followed by Western blot analysis with anti-α6 integrin antibody, AA6A (A). Protein bands in A were quantified and graphed in Excel (B). Data shown were representative of three independent experiments.