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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: Cell Growth Differ. 2002 Mar;13(3):107–113.

Fig. 2.

Fig. 2

Disruption of the actin cytoskeleton reduced total protein expression of α6p but not α6 integrin. Human prostate carcinoma DU145H cells were treated with either 10 μM cytochalasin D (A and B) or 8 μM nocodazole (C and D) over a 24-h period of time. Identical amounts of whole cell lysates (10 μg) were loaded and electrophoresed on a 7.5% polyacrylamide gel under nonreducing conditions. Proteins were transferred to a PVDF membrane followed by Western analysis for α6 integrin (A and C). The α6 and α6p protein bands were scanned and quantitated using Scion Image Analysis software and graphed in Excel (B and D). Data shown were representative of three experiments.