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The α6 integrin sensitivity to endoglycosidase treatment. Human prostate carcinoma DU145H cells were immunoprecipitated for α6 integrin using J1B5 antibody and then subjected to digestion with either endoH or endoF overnight at 37°C. Resulting protein samples were analyzed by 7.5% SDS-PAGE under nonreducing conditions followed by Western blot analysis using anti-α6 integrin antibody, AA6A. The migration of the molecular weight standards and integrins are indicated.
