Table 1.

Primers for multiplex real time PCR for diarrheagenic E. coli genes.

E. coli	Gene	Primer sequence 5-3′*		Size** (bp)	Tm† (C°)
EAEC	aggR	F	CGAAAAAGAGATTATAAAAATTAAC	100	77
		R	GCTTCCTTCTTTTGTGTAT
ETEC	stIa	F	TTTCCCCTCTTTTAGTCAGTCAA	159	81
	stIb	F	TGCTAAACCAGTAGAGTCTTCAAAA	138
	st	R	GCAGGATTACAACACAATTCACAGCAG
	lt	F	TCTCTATGTGCATACGGAGC	322	86
		R	CCATACTGATTGCCGCAAT
EPEC	eaeA	F	ATGCTTAGTGCTGGTTTAGG	248	83
		R	GCCTTCATCATTTCGCTTTC
STEC‡	stx1	F	CTGGATTTAATGTCGCATAGTG	150	87
		R	AGAACGCCCACTGAGATCATC
	stx2	F	GGCACTGTCTGAAACTGCTCC	255	89
		R	TCGCCAGTTATCTGACATTCTG
EIEC	ipaH	F	GTTCCTTGACCGCCTTTCCGATACCGTC	619	91
		R	GCCGGTCAGCCACCCTCTGAGAGTAC
DAEC	daaD	F	TGAACGGGAGTATAAGGAAGATG	444	93
		R	GTCCGCCATCACATCAAAA

^*Primers previously describe by Guion et al¹³;

^**Amplicon size in base pars;

^†Tm, melting temperature for each amplicon;

^‡STEC strains could be eaeA positive and should always have stx1 or stx2 or both stx1+ stx2.