Table 1.
E. coli | Gene | Primer sequence 5-3′* | Size** (bp) | Tm† (C°) | |
---|---|---|---|---|---|
EAEC | aggR | F | CGAAAAAGAGATTATAAAAATTAAC | 100 | 77 |
R | GCTTCCTTCTTTTGTGTAT | ||||
ETEC | stIa | F | TTTCCCCTCTTTTAGTCAGTCAA | 159 | 81 |
stIb | F | TGCTAAACCAGTAGAGTCTTCAAAA | 138 | ||
st | R | GCAGGATTACAACACAATTCACAGCAG | |||
lt | F | TCTCTATGTGCATACGGAGC | 322 | 86 | |
R | CCATACTGATTGCCGCAAT | ||||
EPEC | eaeA | F | ATGCTTAGTGCTGGTTTAGG | 248 | 83 |
R | GCCTTCATCATTTCGCTTTC | ||||
STEC‡ | stx1 | F | CTGGATTTAATGTCGCATAGTG | 150 | 87 |
R | AGAACGCCCACTGAGATCATC | ||||
stx2 | F | GGCACTGTCTGAAACTGCTCC | 255 | 89 | |
R | TCGCCAGTTATCTGACATTCTG | ||||
EIEC | ipaH | F | GTTCCTTGACCGCCTTTCCGATACCGTC | 619 | 91 |
R | GCCGGTCAGCCACCCTCTGAGAGTAC | ||||
DAEC | daaD | F | TGAACGGGAGTATAAGGAAGATG | 444 | 93 |
R | GTCCGCCATCACATCAAAA |
Primers previously describe by Guion et al13;
Amplicon size in base pars;
Tm, melting temperature for each amplicon;
STEC strains could be eaeA positive and should always have stx1 or stx2 or both stx1+ stx2.