Figure 3.

Intracellular concentrations of adenine nucleotides and selected purine salvage pathway metabolites in response to addition of sugar derivatives and different perturbations of energy homeostasis under fermentative and respiratory conditions. Addition of (A–C) 6-deoxyglucose, (D–F) 2-deoxyglucose (each at a final concentration of 110 mmol/l) to trehalose-grown cells. Addition of H₂O₂ (final concentration 2 mM) to (G–I) glucose-grown or (J–L) trehalose-grown cells. (M–O) Addition of antimycin A (final concentration 2 μg/ml) to trehalose-grown cells. (P–R) Transfer of glucose-grown cells to medium containing 110 mmol/l trehalose as the only carbon source. Cells were cultivated on glucose until mid-exponential phase, spun down by centrifugation, and resuspended in trehalose medium having the same composition except for the carbon source. (Cells that were resuspended in glucose medium as a control did not exhibit any change in AXP concentration; data not shown.) All experiments were carried out using wild-type cells. Left panel of figures: ATP, ADP, and AMP. Middle panel of figures: hypoxanthine, inosine, and IMP. Right panel of figures: Sum of AXP nucleotides, sum of (AXP+IMP+inosine+hypoxanthine). Data represent the average of at least two independent experiments.