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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: J Orthop Res. 2009 Dec;27(12):1612–1618. doi: 10.1002/jor.20932

Table 1.

Decellularization Protocols

Trypsin SDS TRITON-X Control
0.1% Trypsin 0.1% SDS 0.25% TRITON-X PBS −/−
0.02% EDTA PBS −/− 0.25% NA-deoxycholate
PBS −/− PBS −/−
48h 24h 24h at 37°C
wash w/ medium 72h/4°C
All decellularization procedures were concluded with:
Ribonuclease (RNase 100 μg/mL)
Deoxyribonuclease (DNase 150 IU/mL)
24h at 37°C
wash w/PBS and 0.02% EDTA for 24h at 4°C