A) His6 tagged yHsp90 was precipitated from untreated or GA-treated (100 μM, 1 h) yeast cells and its tyrosine phosphorylation was detected with anti-phosphotyrosine antibody.
B) Deletion of SWE1 abolishes tyrosine phosphorylation of yHsp90.
C) Over-expression of GST tagged Swe1 in swe1Δ cells restores tyrosine phosphorylation of yHsp90. Swe1-GST was detected by anti-GST antibody.