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. Author manuscript; available in PMC: 2011 Feb 12.
Published in final edited form as: Mol Cell. 2010 Feb 12;37(3):333–343. doi: 10.1016/j.molcel.2010.01.005

Figure 1. Swe1 phosphorylates yHsp90.

Figure 1

A) His6 tagged yHsp90 was precipitated from untreated or GA-treated (100 μM, 1 h) yeast cells and its tyrosine phosphorylation was detected with anti-phosphotyrosine antibody.

B) Deletion of SWE1 abolishes tyrosine phosphorylation of yHsp90.

C) Over-expression of GST tagged Swe1 in swe1Δ cells restores tyrosine phosphorylation of yHsp90. Swe1-GST was detected by anti-GST antibody.