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D-cyclin suppression induces senescence. (A) Representative images show transduced YFP expressing PANC1 cells, 20 days post-transduction. Arrows indicate YFP cells of large senescent-like morphology that were quantified (B), bars are mean ± standard error. (C) A representative image shows that larger cells of shD3_1 transduced PANC1 display senescence associated β-galactosidase activity (arrows). Scale bars designate 20 μm.
