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pIX-Luc expression in a stable cell line. pIX-Luc expression was determined in a stable cell line expressing pIX-Luc via Western blot analysis. 10 μg of protein was boiled in Laemmli sample buffer for 5 minutes and resolved on 4 to 15% SDS-PAGE and transferred to PVDF membrane. Western blot analysis was performed with an anti-Flag antibody. Signal was visualized with enhanced chemiluminescence according to the manufacture's instructions. The solid arrow indicates pIX-Luc.
