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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):7233–7243. doi: 10.4049/jimmunol.0801375

FIGURE 6.

FIGURE 6

Effect of FK506 and DEX on the translocation of NF-κB and NF-AT by anti-IgE treatment in human mast cells. IgE-sensitized human mast cells were preincubated with either 1 μM DEX, 100 nM FK506, or 0.01% DMSO for 1 h and then stimulated with 1.5 μg/ml anti-IgE Ab for 30 min. A, Immunofluorescence images were showing the distribution of NF-κB and NF-AT. Mast cells were treated with mouse anti-NF-κB p65 and rabbit anti-NF-ATc3 for localization of endogenous NF-κB (green fluorescence) and NF-AT (red fluorescence). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue fluorescence). The images are representative of four independent preparations. B, Summary of the percentage of mast cells in which NF-κB p65 (upper panel) and NF-ATc3 (lower panel) has localized within the nuclei. The results are shown as the means ± SEM of four independent experiments.*, p < 0.05.