Figure 7.

ebpA expression affected by NaHCO₃, and not CO₂. For β-gal assays, samples were collected every hour from 3 to 8 hr, then at 10 and 24 hr after starting the culture (x axis). Growth curves of OG1RF containing P_ebpA::lacZ are shown in air with a thin gray line, in NaHCO₃/air with thin orange line, in CO₂ with a dense gray line, and in NaHCO₃/CO₂ with a dense orange line. The β-gal assays for OG1RF containing P_ebpA::lacZ are represented with closed black square, closed orange square, open black square, and open orange square when the cells were grown in air, 5% CO₂, NaHCO₃-air, and NaHCO₃-5% CO₂, respectively. All sets of cultures presented were analyzed concurrently. This figure is a representative of at least two experiments. A. OD_{600 nm} readings. B. β-gal assays (β-gal units = OD_{420 nm}/protein concentration in mg/ml).